Cameos, in-jokes, re-used animation and other trivia for Madagascar: Escape 2 Africa.
- Bernie Mac's last animated feature. This movie was dedicated to him.
- The sequel Madagascar 3 (2012) was confirmed months before this one came out.
- 'Moto Moto' means 'Hot Hot' in Swahili.
- The line the English penguin delivers in his safety speech on the plane is the same safety tip that the rat gives the chickens in the Aardman film, Chicken Run (2000).
- The print on Alex and Zuba's paw are both the shape of Africa.