- The first two movie traliers had Jerry Seinfeld trying to make a live action Bee Movie After watching the disastrous results Steven Spielberg asks Why don't you just make it a cartoon.
- In some areas release prints were delivered to theaters with fake titles Flower and Jocks.
- According to Jerry Seinfeld while haing lunch at Steven Spielberg's house he first mentioned the idea for the movie as a joke but Spielberg loved the idea.
- In the first scene they showed a collection of white sneakers that Barry owns In real life Jerry Seinfeld is said to have a collection of more than 500 sneakers and they are all white.
- Two references to The Beatles include the song Here Comes the Sun and the Flight Controller saying Coo- Coo-Ca-Choo as the plane flies and settles in a bee-like fashion.
- The actual address of the fictitious Vanessa's Flower Shop is at the corner of 67th W and Columbus New York New York.
- Fozzie Bear from The Muppet Show is mentioned when the owner of Honey Farms talks about bears being loveable creatures. He says "Yogi Bear, Fozzie Bear, Build-A-Bear."
- Pooh and Piglet make a cameo during the movie.